Platelet-Rich Plasma (PRP) is a low-cost procedure to deliver high concentrations of autologous growth factors (GFs). Platelet\nactivation is a crucial step that might influence the availability of bioactive molecules and therefore tissue healing. Activation of\nPRP from ten voluntary healthy males was performed by adding 10% of CaCl2, 10% of autologous thrombin, 10% of a mixture\nof CaCl2 + thrombin, and 10% of collagen type I. Blood derivatives were incubated for 15 and 30 minutes and 1, 2, and 24 hours\nand samples were evaluated for the release of VEGF, TGF-
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